An Automated High-Throughput Counting Method for Screening Circulating Tumor Cells in Peripheral Blood - Analytical Chemistry (ACS Publications)
Summary:
This is a very unique paper that presents direct labelling of cells in whole blood. besides the traditional EpCAM, CD4-, CK markers, the authors also used CD44+ marker for stemness.
methods:
1 ml of blood directly stained with markers, centrifuged to remove excess labelling antibody and resuspended and flown through the microfluidic chip. A custom built line-confocal imaging system captures images of cells flowing past the microfluidic channel.
authors had previously published a variation of this technique here http://www.ncbi.nlm.nih.gov/pubmed/22389033
performance:
Summary:
This is a very unique paper that presents direct labelling of cells in whole blood. besides the traditional EpCAM, CD4-, CK markers, the authors also used CD44+ marker for stemness.
methods:
1 ml of blood directly stained with markers, centrifuged to remove excess labelling antibody and resuspended and flown through the microfluidic chip. A custom built line-confocal imaging system captures images of cells flowing past the microfluidic channel.
authors had previously published a variation of this technique here http://www.ncbi.nlm.nih.gov/pubmed/22389033
performance:
- 94% recovery
- 1 ml of blood processed in ~ 30 mins
- no isolation/enrichment needed
interestingly, the performance of this system was compared with cellsearch in 90 clinical samples with the following findings
91% positive samples vs 44% positive using cellsearch in 7.5 ml of blood
range of cells 15 to 3375 per 7.5ml vs 1 to 846 by cellsearch
average 305 cells/ml vs 36 cells/ml by cellsearch
very impressive performance improvement over cellsearch.
No comments:
Post a Comment