A list of all relevant patents for "rare cells from fluid" can be found here
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http://www.google.com/patents/US7863012
http://www.google.com/patents/EP1425294B1?cl=en
http://www.google.com.sg/patents/EP1597353A2?cl=en
http://www.google.com.sg/patents/US8329422
Technology: EpCAM based enrichment using magnetic beads
Abstract:
The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.
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(Company: Veridex LLC)
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http://www.google.com.sg/patents/EP2229441A2?cl=en
Technology: EpCAM based moagentic beads
Abstract:
Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
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http://www.google.com.sg/patents/EP1984030A4?cl=en
Technology: HD CTC technology
Abstract:
Methods are provided for detecting circulating tumor cells in a mammalian subject. Methods of diagnosing cancer in a mammalian subject are provided. The methods of detection or diagnosis indicate the presence of metastatic cancer or early stage cancer.
(Company: Scripps institute (EPIC Sciences))
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http://www.google.com.sg/patents/EP1874920A2?cl=en
Technology:
Abstract:
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
(Company: CellPoint diagnostics)
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http://www.google.com.sg/patents/US20110294186
Technology:
Abstract:
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
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(Company: On-Q-ity)
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http://www.google.com.sg/patents/US7993821
http://www.google.com.sg/patents/US20120178097
Technology:
Abstract:
Embodiments in accordance with the present invention relate to methods and apparatuses for concentrating and isolating Circulating Tumor Cells (CTCs) from body fluids. One embodiment of the present invention includes a micro-fabricated or nano-fabricated device having channels configured for separating and excluding. Embodiments in accordance with the present invention utilize features that reduce the hydrodynamic pressure experienced by the cells during the separation, isolation and concentration processes, and therefore reduce the likelihood of cell lysis or other damage to the cells..
(Company: Daniel Chiu, U Washington)
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http://www.google.com.sg/patents/US20120100521
Technology:
Abstract:
Microdevices are disclosed to efficiently, accurately, and rapidly isolate and enumerate rare cells, such as circulating tumor cells, from liquids such as whole blood. The system employs multiple parallel meandering channels having a width on the order of 1-2 cell diameters. The microdevices can be produced at low-cost, may readily be automated, and in many instances may be used without pre-processing of the sample. They may be used to isolate and enumerate rare cells, including for example the detection and diagnosis of cancers, cancer staging, or evaluating the effectiveness of a therapeutic intervention, or detecting pathogenic bacteria. The device may optionally be used to nondestructively capture and later to release target cells.
(Company: Steven Soper, Biofluidica)
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http://www.google.com/patents/EP2201361B1?cl=en
Technology: multiple antibodies on magnetic beads
Abstract:
The present invention relates to the detection of tumor stem cells and tumor cells in epithelial-mesenchymal transition and uses of such methods. According to the present invention said method comprises a selecting step for selection or enrichment of said predetermined cells from the sample wherein the sample is contacted with the solid surface for preferential binding of said predetermined cells to the solid surface and then the sample is removed from the solid surface in a washing step. The inventive method is characterized in that the sample contains a polyol at least during one of contacting the sample with the solid surface and the washing step and in a detection step detecting in said cells, preferentially selected or enriched by said selecting step, the presence or absence of expression of at least one marker associated with at least one of the group comprising tumor stem cells and tumor cells in epithelial-mesenchymal transition.
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http://www.google.com/patents/US7846393
Technology: 3D parylene membrane filter for isolation of CTC
Abstract:
The present invention provides a parylene-based membrane filter device for capturing of circulating tumor cells (CTC). The membrane filter has an array of holes having a predetermined geometric design with precisely controlled size, shape and density. In one aspect, the device has a stack of substantially parallel membrane filters with uniformly-spaced and/or monodispersed holes.
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(Company: Filtini)
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http://www.google.com.sg/patents/US8088715
Technology: automated microscope
Abstract:
An automated, highly sensitive, specific and potentially quantitative detection method using an automated microscope for identifying and enumerating rare cancer cells in blood and other fluids.
(Company: Ikonisys)
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http://www.google.com/patents/US6949355
http://www.google.com.sg/patents/EP2041299A2?cl=en
Technology: negative enrichment of CTCs
Abstract:
The invention provides methods and compositions for isolating and detecting rare cells from a sample containing other types of cells. In particular, the invention includes a debulking step that can use a microfabricated filter or a cell lysis step or density-based methods to remove certain types of cells, and immobilization methods to remove other types of cells. The invention also includes a method for determining the number or proportion of cancer cells in a biological sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample, resulting in the enrichment of rare target cells. The presence or level of target cells provides information about various physiological conditions, such as cancer
(Company: Aviva Biosciences)
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http://www.google.com.sg/patents/US20070026413
Technology: microposts coated with EpCAM
Abstract:
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
(Company: Mehmet Toner, Harvard)
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http://www.google.com.sg/patents/US20070026419
Technology: Materal displacement obstacle chip
Abstract:
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
(Company: Martin Fuchs, Cellective Dx)
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http://www.google.com/patents/US8263404
Technology: RBC agglutination followed by filtration
Abstract:
An antigen-dependent negative selection blood cell separation method is described. Rare circulating epithelial cells can be separated from blood by depleting erythrocytes from a blood sample. Erythrocytes are depleted by agglutination. The new method comprises the use of an agglutinating agent, such as an anti-glycophorin A or glycophorin B antibody, as glycophorin A or B are present on erythrocytes and not on the desired epithelial cells. With regular mixing, desired rare circulating epithelial cells do not become entrapped in the red cell agglutinate.
(Company: Medical Discovery partners)
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http://www.google.com.sg/patents/US20120100560
Technology: anti-body coated microfluidic chip and quantum dots-based imagingi
Abstract:
Applications in nanomedicine, such as diagnostics and targeted therapeutics, rely on the detection and targeting of membrane biomarkers. The present invention, in one embodiment, utilizes quantitative profiling, spatial mapping, and multiplexing of cancer biomarkers using functionalized quantum dots. This approach provides highly selective targeting molecular markers for pancreatic cancer with extremely low levels of non-specific binding and provides quantitative spatial information of biomarker distribution on a single cell, which is important since tumors cell populations are inherently heterogeneous. The quantitative measurements (number of molecules per square micron) is validated using flow cytometry and demonstrated using multiplexed quantitative profiling using color-coded quantum dots.
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(Company: johns Hopkins university)
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http://www.google.com/patents/US8263404
Technology: RBC agglutination followed by filtration
Abstract:
An antigen-dependent negative selection blood cell separation method is described. Rare circulating epithelial cells can be separated from blood by depleting erythrocytes from a blood sample. Erythrocytes are depleted by agglutination. The new method comprises the use of an agglutinating agent, such as an anti-glycophorin A or glycophorin B antibody, as glycophorin A or B are present on erythrocytes and not on the desired epithelial cells. With regular mixing, desired rare circulating epithelial cells do not become entrapped in the red cell agglutinate.
(Company: Medical Discovery partners)
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WO2010/144745 A2
http://www.sumobrain.com/patents/wipo/Sheath-flow-devices-methods/WO2010144745.html
Technology: Microfluidic cheath flow for cell isolation
Abstract: The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products
(Company: Cynvenio Biosystems)
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US2011/0070642 A1
http://www.patentlens.net/patentlens/patents.html?patnums=US_2011_0070642_A1&language=&
Abstract: A device for isolating and cultivating live cells on a filter or for extracting the genetic material thereof includes: a filter holder (108) connected to a filter; a compartment (102) having an upper opening and a lower opening; and an element (110) that is mobile relative to the compartment for applying a force on the holder and releasing the holder. According to the embodiments, the filter holder is mechanically connected to the compartment or to the mobile element until the application of the force. Preferably, the device further includes a removable end piece (104) tightly and removably attached and adapted for preventing the relative movement of the mobile element and the compartment for applying the force and releasing the holder.
(Assignee: ScreenCell)
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20120258475
Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
(Assignee: Biocept)
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